Activation of diacylglycerol O‐acyltransferase 1 gene results in increased tumor necrosis factor‐α gene expression in 3T3‐L1 adipocytes

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Abstract
The tumor necrosis factor‐α (TNF‐α) expression has been reported to be largely dependent on the size of adipocytes. We herein investigated the gene regulation of diacylglycerol O‐acyltransferase (DGAT) in order to clarify the mechanism of TNF‐α expression induced in large adipocytes. 3T3‐L1 cells were cultured in the presence of 5 mM or 25 mM glucose to generate adipocytes from which the triglyceride content differs. The expression of TNF‐α, DGAT1, and DGAT2 were upregulated in adipocytes cultured with 25 mM glucose. Furthermore, knockdown of DGAT1 gene significantly inhibited the TNF‐α expression. Finally, the DGAT1 expression levels were closely related to the TNF‐α level in 3T3‐L1 adipocytes.