Characterization of the Glycosyltransferase Activity of DesVII: Analysis of and Implications for the Biosynthesis of Macrolide Antibiotics
- 5 May 2004
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 126 (21) , 6534-6535
- https://doi.org/10.1021/ja049967j
Abstract
In vitro catalytic activity of DesVII, the glycosyltransferase involved in the biosynthesis of methymycin, neomethymycin, narbomycin, and pikromycin in Streptomyces venezuelae, is described. This is the first report of demonstrated in vitro activity of a glycosyltransferase involved in the biosynthesis of macrolide antibiotics. DesVII is unique among glycosyltransferases in that it requires an additional protein component, DesVIII, as well as basic pH for its full activity.Keywords
This publication has 1 reference indexed in Scilit:
- Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.Published by Elsevier ,2003