TECHNIQUES FOR THE SEPARATION OF SPECIFIC MURINE ISOANTIBODIES FROM SERUM
- 1 February 1970
- journal article
- research article
- Published by Wolters Kluwer Health in Transplantation
- Vol. 9 (2) , 151-157
- https://doi.org/10.1097/00007890-197002000-00008
Abstract
Antisera prepared against histocompatibility (H-2) antigens were absorbed with erythrocyte stromata from mice of the inbred strains A (H-2a), A:SW (H-2s), BALB/c (H-2d), DBA/2 (H-2d), C3H (H-2k), C57BL (H-2b), and RFM (H-2f). Hemagglutinating antibody was eluted from the stroma of each strain with glycine-sulfate buffer. By selection of strains having different H-2 specificities, it was possible to successively remove and recover, from a multispecific starting material, antibodies having an apparently single specificity.Keywords
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