Transforming Growth Factor-α in Human Submandibular Gland and Saliva
- 1 November 1995
- journal article
- research article
- Published by Taylor & Francis in Journal of Immunoassay
- Vol. 16 (4) , 379-394
- https://doi.org/10.1080/15321819508013569
Abstract
A sensitive sandwich enzyme immunoassay (EIA) for transforming growth factor-α (TGF-α) utilizing a polyclonal antibody that recognizes limited epitopes of both human TGF-α and rat TGF-α in combination with a monoclonal anti-TGF-α IgG1 galactosidase conjugate was developed. This assay shows no cross-reactivity with human epidermal growth factor. We can quantify the TGF-α level in not only human TGF-α (detection limit: 1 pg/ml), but also rat TGF-α (detection limit: 10 pg/ml) by virtue of cross-reactivity. Employing this assay system, we demonstrated that TGF-α is present in both human submandibular glands and submandibular/sublingual saliva.Keywords
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