In Vitro Cultivation of the Vascular Phase of Sarcocystis capracanis and Sarcocystis tenella1

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Abstract
Sporozoites of Sarcocystis capracanis and S. tenella (Apicomplexa) penetrated all four cell types tested (bovine monocytes, BM; bovine pulmonary artery endothelial cells, CPA; Madin‐Darby bovine kidney; and ovine monocytes). Sporozoites of S. tenella developed to meronts in BM and CPA; those of S. capracanis developed to meronts in BM only. Both species of Sarcocystis developed to large first‐generation meronts followed by small meronts. At 40 to 50 days after inoculation (DAI) of sporozoites, considerably more merozoites of S. tenella were harvested from CPA (24.9 × 106 merozoites/75‐cm2 flask; n = 4) than from BM (1.9 × 106 merozoites/75‐cm2 flask; n = 4). Merozoites of S. capracanis were most numerous in BM at 88 to 100 DAI during which time 2.1 × 106 merozoites/75‐cm2 flask (n = 4) were harvested.