Sites of Gibberellin Biosynthesis in Pea Seedlings
- 1 July 1985
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 78 (3) , 655-657
- https://doi.org/10.1104/pp.78.3.655
Abstract
Potential sites of gibberellin biosynthesis in 10-day-old `Alaska' pea (Pisum sativum L.) seedlings were investigated using a cell-free ezyme system capable of incorporating [14C]-mevalonic acid into ent-kaurene. In peas, ent-kaurene is assumed to be a committed intermediate in the gibberellin biosynthetic pathway. Comparative results from enzyme assays using extracts from shoot tips, leaf blades, internodes, and root tips indicate that the highest capacity for ent-kaurene (and presumably gibberellin) synthesis is in those tissues with the greatest potential for growth. The highest rates were obtained with extracts prepared from the fifth (youngest) internode, the fourth (youngest) expanded leaf, and the shoot tip itself. This report represents the first direct evidence that the enzymes responsible for early stages in gibberellin biosynthesis occur in internode tissues with potential for rapid elongation.Keywords
This publication has 11 references indexed in Scilit:
- Isolation of a Kaurene Synthetase Inhibitor from Castor Bean Seedlings and Cell Suspension CulturesPlant Physiology, 1981
- Analysis of Native Gibberellins in the Internode, Nodes, Leaves, and Inflorescence of Developing Avena PlantsPlant Physiology, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Correlations of Growth Rate and De-etiolation with Rate of Ent-Kaurene Biosynthesis in Pea (Pisum sativum L.)Plant Physiology, 1974
- Apparent Changes in Rate of Kaurene Biosynthesis During the Development of Pea SeedsPlant Physiology, 1969
- Extractable and Diffusible Gibberellins From Light- and Dark-grown Pea SeedlingsPlant Physiology, 1968
- Biosynthesis of (—)-Kaurene in Cell-free Extracts of Immature Pea SeedsPlant Physiology, 1967
- Organs of Gibberellin Synthesis in Light-Grown Sunflower PlantsPlant Physiology, 1966
- Gibberellin Production in Pea Seeds Developing in Excised Pods: Effect of Growth Retardant AMO-1618Science, 1965
- Studies on the Organ of Production of the Natural Gibberellin Factor in Higher Plants.Plant Physiology, 1957