Combined sacB -Based Negative Selection and cre-lox Antibiotic Marker Recycling for Efficient Gene Deletion in Pseudomonas Aeruginosa
Open Access
- 1 January 2005
- journal article
- research article
- Published by Taylor & Francis in BioTechniques
- Vol. 38 (1) , 63-67
- https://doi.org/10.2144/05381st01
Abstract
The complete genome of the bacterial pathogen Pseudomonas aeruginosa has now been sequenced, allowing gene deletion, one of the most frequently used methods in gene function study, to be fully exploited. In this study, we combine...Keywords
Funding Information
- the association “Vaincre la Mucoviscidose.”
This publication has 14 references indexed in Scilit:
- VEX-capture: a new technique that allows in vivo excision, cloning, and broad-host-range transfer of large bacterial genomic DNA segmentsJournal of Microbiological Methods, 2004
- Broad-Host-Range cre-lox System for Antibiotic Marker Recycling in Gram-Negative BacteriaBioTechniques, 2002
- Children with Cystic Fibrosis Produce an Immune Response against Exoenzyme S, a Type III Cytotoxin ofPseudomonas aeruginosaThe Journal of Infectious Diseases, 2002
- Type III Protein Secretion Is Associated with Death in Lower Respiratory and SystemicPseudomonas aeruginosaInfectionsThe Journal of Infectious Diseases, 2001
- Sera from Adult Patients with Cystic Fibrosis Contain Antibodies to Pseudomonas aeruginosa Type III ApparatusInfection and Immunity, 2001
- An improved system for gene replacement and xylE fusion analysis in Pseudomonas aeruginosaGene, 1995
- Pseudomonas aeruginosa Contains an IHF-like Protein That Binds to the algD PromoterBiochemical and Biophysical Research Communications, 1993
- Precise Deletions in Large Bacterial Genomes by Vector-mediated Excision (VEX)Journal of Molecular Biology, 1993
- A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative BacteriaBio/Technology, 1983
- Bacteriophage P1 site-specific recombinationJournal of Molecular Biology, 1981