Purification of the pro-phenol oxidase enzyme from haemocytes of the cockroach Blaberus discoidalis
- 1 January 1993
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 289 (1) , 87-91
- https://doi.org/10.1042/bj2890087
Abstract
Pro-phenol oxidase was purified from the haemocytes of the cockroach Blaberus discoidalis by Blue Sepharose chromatography, hydrophobic-interaction chromatography on a Phenyl-Superose column and, finally, gel filtration on a Superose 6 column. Results suggest that the molecule exists as a polymer of identical 76 kDa monomeric units. The enzyme is a glycoprotein with pI of 5.2 and can be converted by trypsin into phenol oxidase.Keywords
This publication has 22 references indexed in Scilit:
- Analysis of Carbohydrate Properties Essential for Melanogenesis in Tyrosinases of Cultured Malignant Melanoma Cells by Differential Carbohydrate Processing InhibitionJournal of Investigative Dermatology, 1990
- Granular phenoloxidase involved in cuticular melanization in the tobacco hornworm: Regulation of its synthesis in the epidermis by juvenile hormoneDevelopmental Biology, 1988
- Formation and the inhibition of reaction plug in mated Drosophila — Study of a primitive defense reactionDevelopmental & Comparative Immunology, 1988
- Protease inhibitor controls prophenoloxidase activation in Manduca sextaFEBS Letters, 1986
- A Rapid Method for Detection of Tyrosinase Activity in ElectrophoresisStain Technology, 1986
- Hemocyte lysate enhancement of fungal spore encapsulation by crayfish hemocytesDevelopmental & Comparative Immunology, 1984
- Activation of pro-phenoloxidase by bacterial cell walls or β-1,3-glucans in plasma of the silkworm, Bombyx moriBiochemical and Biophysical Research Communications, 1983
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Purification of Prophenoloxidase in the Haemolymph ofCalliphora vicina(R. & D.)Archives Internationales de Physiologie et de Biochimie, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970