A COMPARISON OF METHODS FOR THE ISOLATION OF FUNGI FROM RHIZOSPHERES

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Abstract
A comparison was made between the fungi isolated from non-rhizosphere soil and from rhizosphere soil of mature dwarf bean plants by three methods of isolation: soil dilution plating, soil plating, and soil washing followed by the plating of washed soil particles. The data obtained with rhizosphere soil demonstrated that the use of the soil-washing technique allowed an increased frequency of isolation of sterile forms, Fusarium spp., and members of the Mucorales, whereas heavily sporing forms were isolated with decreased frequency. It is concluded that, compared with other isolation methods, the soil-washing technique allows a more accurate assessment of fungi active in the rhizosphere.

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