Virus-specified Polypeptides in Cells Infected with Semliki Forest Virus

Abstract
Summary Five polypeptide components were resolved by polyacrylamide gel electrophoresis of the proteins extracted from cells infected with Semliki Forest virus. One of these components was the virus nucleocapsid polypeptide, another was the two unresolved virus envelope glycoproteins. The remaining three peaks were due to polypeptides of mol. wt. 97000 (NVP-97), 78000 (NVP-78) and 63000 (NVP-63). NVP-97 and NVP-63 are glycoproteins, and both NVP-63 and the envelope glycoproteins were precipitated with either an antiserum to the envelope polypeptides or with concanavalin A.

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