Identification ofDekkera bruxellensis(Brettanomyces) from Wine by Fluorescence In Situ Hybridization Using Peptide Nucleic Acid Probes

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Abstract
A new fluorescence in situ hybridization method using peptide nucleic acid (PNA) probes for identification ofBrettanomycesis described. The test is based on fluorescein-labeled PNA probes targeting a species-specific sequence of the rRNA ofDekkera bruxellensis. The PNA probes were applied to smears of colonies, and results were interpreted by fluorescence microscopy. The results obtained from testing 127 different yeast strains, including 78Brettanomycesisolates from wine, show that the spoilage organismBrettanomycesbelongs to the speciesD. bruxellensisand that the new method is able to identifyBrettanomyces(D. bruxellensis) with 100% sensitivity and 100% specificity.