Induction of protoplast-like cells in Bifidobacterium bifidum Es5. I. Induction of protoplasts and L-forms by penicillin.

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Abstract
A method for protoplast induction with penicillin G (PCG) in B. bifidum Es5 was examined. Growing cells were converted into protoplasts in a nutrient broth containing PCG without addition of any osmotic stabilizers. The maximal rate of protoplast induction was .apprx. 50% after 6 h incubation with 1 U PCG/ml. Supplementing the PCG-treatment culture with 0.1-0.2 M sucrose raised the induction rate to 80% or more. Sucrose may make the bacteria more sensitive to PCG and subsequently stabilize the protoplasts. Sucrose by itself caused a decrease in the number of viable cells but not in the turbidity of the culture without PCG. L-colonies of strain Es5 developed from bacterial suspensions and also from protoplast suspensions inoculated on nutrient agar plates containing PCG alone or PCG and sucrose.

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