Influence of long‐term repeated prescribed burning on mycelial communities of ectomycorrhizal fungi
Open Access
- 5 June 2006
- journal article
- Published by Wiley in New Phytologist
- Vol. 172 (1) , 149-158
- https://doi.org/10.1111/j.1469-8137.2006.01793.x
Abstract
• To demonstrate the efficacy of direct DNA extraction from hyphal ingrowth bags for community profiling of ectomycorrhizal (ECM) mycelia in soil, we applied the method to investigate the influence of long-term repeated prescribed burning on an ECM fungal community. • DNA was extracted from hyphal ingrowth bags buried in forest plots that received different prescribed burning treatments for 30 yr, and denaturing gradient gel electrophoresis (DGGE) profiles of partial fungal rDNA internal transcribed spacer (ITS) regions were compared. Restriction fragment length polymorphism (RFLP) and sequence analyses were also used to compare clone assemblages between the treatments. • The majority of sequences derived from the ingrowth bags were apparently those of ECM fungi. DGGE profiles for biennially burned plots were significantly different from those of quadrennially burned and unburned control plots. Analysis of clone assemblages indicated that this reflected altered ECM fungal community composition. • The results indicate that hyphal ingrowth bags represent a useful method for investigation of ECM mycelial communities, and that frequent long-term prescribed burning can influence below-ground ECM fungal communities.Keywords
This publication has 41 references indexed in Scilit:
- A ‘dirty’ business: testing the limitations of terminal restriction fragment length polymorphism (TRFLP) analysis of soil fungiMolecular Ecology, 2006
- Assemblages of ericoid mycorrhizal and other root‐associated fungi from Epacris pulchella (Ericaceae) as determined by culturing and direct DNA extraction from rootsEnvironmental Microbiology, 2005
- Experimental warming and burn severity alter soil CO2flux and soil functional groups in a recently burned boreal forestGlobal Change Biology, 2004
- Diversity and ecology of soil fungal communities: increased understanding through the application of molecular techniquesEnvironmental Microbiology, 2004
- Diversity of fungi in organic soils under a moorland – Scots pine (Pinus sylvestris L.) gradientEnvironmental Microbiology, 2003
- Long‐term increase in nitrogen supply alters above‐ and below‐ground ectomycorrhizal communities and increases the dominance ofRussulaspp. in a temperate oak savannaNew Phytologist, 2003
- CANONICAL ANALYSIS OF PRINCIPAL COORDINATES: A USEFUL METHOD OF CONSTRAINED ORDINATION FOR ECOLOGYEcology, 2003
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Fire and PlantsPublished by Springer Nature ,1996
- Microbial community structure and pH response in relation to soil organic matter quality in wood-ash fertilized, clear-cut or burned coniferous forest soilsSoil Biology and Biochemistry, 1995