Preparation of Human Plasminogen Suitable as Substrate for Quantitative Determination of Plasminogen Activators
- 1 January 1966
- journal article
- schattauer gmbh
- Published by Georg Thieme Verlag KG in Thrombosis and Haemostasis
- Vol. 15 (03/04) , 511-518
- https://doi.org/10.1055/s-0038-1649453
Abstract
A simple method for preparation of plasminogen with low spontaneous activity and soluble at a neutral pH and at physiological ionic strength is described. Euglobulin made from fresh, oxalated, BaSO4-adsorbed, human plasma was first purified by means of gel filtration on Sephadex G-200. After gel filtration, further purification and concentration was done on DEAE-sephadex A-50. The activity was 100-130 casein units per mg tyrosine.Keywords
This publication has 7 references indexed in Scilit:
- Human and Bovine Plasminogen-Free Thrombin, Purified by Means of Gel Filtration and Ion Exchange ChromatographyThrombosis and Haemostasis, 1966
- The purification and properties of human plasminogenBiochemical Journal, 1964
- Further studies with human plasminogenBiochemical Journal, 1962
- Purification of human plasminogenBiochemical Journal, 1962
- The assay of human plasminogen with casein as substrateBiochemical Journal, 1961
- ε-Aminocaproic Acid: an Inhibitor of Plasminogen ActivationJournal of Biological Chemistry, 1959
- Effect of Lysine on the Purification of Human Plasminogen on Cellulose Ion Exchangers.Acta Chemica Scandinavica, 1959