The Determination of Charge of Anionic Tc-99m Radiopharmaceuticals
- 1 August 1992
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 15 (12) , 2165-2174
- https://doi.org/10.1080/10826079208016332
Abstract
The absolute charges on several anionic Tc-99m complexes were determined by an HPLC method using a SAX column and sodium sulphate as eluent. Retention times were measured over a range of [SO4 2-], and a non-linear regression technique was employed to determine complex charge. The system was validated using pertechnetate (-1) and the ligand TDG (-2). The charge of Tc-99m DTPA was shown to be -2. The major Tc-99m EHIDA complex has a charge of -1, while the main transient complex of this ligand has a -2 charge. Both of the complexes of Tc-99m TDG have a charge of -2. The method provided near integer values in all cases.Keywords
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