Evidence that the axolemmal mitogen for cultured schwann cells is a positively charged, heparan sulfate proteoglycan‐bound, heparin‐displaceable molecule

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Abstract
Treatment of axolemma with pH 9 buffer results in a pellet enriched two‐fold in the mitogen for cultured Schwann cells. Heparitinase treatment releases 8% of the mitogen into solution, while heparin selectively solubilizes the mitogen, resulting in an extract which has a specific mitogenic activity approximately 2.5 times greater than the mitogenicity of the starting axolemma membrane. These data support a model in which the axolemmal mitogen is a positively charged molecule associated with negatively charged sulfated proteoglycans.