Proluminal Movement of 3H-Androgen across the Epididymal Epithelium in the Rat after Hypophysectomy and Gonadotropin Supplementation1

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Abstract
The effects of hypophysectomy and gonadotropin replacement on transepithelial movement of 3H-androgen in the rat epididymis were examined by in vivo microperifusion of 3H-testosterone followed by in vivo micropuncture to obtain peritubular and intraluminal fluid. In the caput epididymidis of normal rats, intraluminal 3H-androgen concentrations were approximately 300% of those in the interstitial space. In contrast, proluminal movement of 3H-androgen into rat caput epididymal tubules was significantly decreased 10 days after hypophysectomy. 3H-Testosterone movement across the caput epididymal epithelium was completely returned to normal by supplementation with 24 .mu.g/day follicle-stimulating hormone (FSH) or 24 .mu.g/day luteinizing hormone (LH). However, neither 0.12 .mu.g/day FSH nor 250 .mu.g/day prolactin returned proluminal androgen movement to normal. It is speculated that epididymal uptake of peritubular testosterone is mediated by androgen-binding protein, which is known to be secreted by Sertoli cells after stimulation by FSH or testosterone.