Stage- and tissue-specific expression of the genes encoding calliphorin, the major larval serum protein of Calliphora vicina
- 1 July 1986
- journal article
- Published by Springer Nature in Wilhelm Roux' Archiv für Entwicklungsmechanik der Organismen
- Vol. 195 (5) , 290-295
- https://doi.org/10.1007/bf00376061
Abstract
The stage- and tissue-specific biosynthesis of calliphorin was analysed during the development of the blowfly, Calliphora vicina. Western blot analyses show that the protein is not present in eggs, whereas it can be detected in fat body, brain, imaginai disk, salivary gland and epidermis throughout all postembryonic stages, including the adult one. By Northern analysis a unique 2.6 kb mol.wt. mRNA coding for calliphorin is identified exclusively in the fat body tissue of larvae, pupae and adults. Hybridization experiments of in vivo labelled poly(A)+ RNA with filter-bound calliphorin genes indicate that the genes are transcribed until pupariation. However, the translation of the calliphorin mRNA stops at the end of the feeding stage, as shown by [35S]-methionine incorporation.Keywords
This publication has 14 references indexed in Scilit:
- Regulation of synthesis of the larval serum proteins of Drosophila melanogasterDevelopmental Biology, 1984
- Immunocytochemical and electrophoretic studies on the localization of the major haemolymph proteins (ceratitins) inCeratitis capitata during developmentWilhelm Roux' Archiv für Entwicklungsmechanik der Organismen, 1983
- Ecdysteroids regulate the synthesis of RNA polymerase molecules in fat body cells ofCalliphora larvaeWilhelm Roux' Archiv für Entwicklungsmechanik der Organismen, 1983
- Translation of the mRNAs coding for the major hemolymph proteins of Ceratitis capitata in cell-free system: Comparison of the translatable mRNA levels to the respective biosynthetic levels of the proteins in the fat body during developmentDevelopmental Biology, 1983
- Major haemolymph proteins inCeratitis capitata: Biosynthesis and secretion during developmentWilhelm Roux' Archiv für Entwicklungsmechanik der Organismen, 1981
- Calliphorin, a major protein of the blowfly: Correlation between the amount of protein, its biosynthesis, and the titer of translatable calliphorin-mRNA during developmentDevelopmental Biology, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Synthesis of poly(A) containing RNA induced by ecdysterone in fat body cells of Calliphora vicinaJournal of Insect Physiology, 1977
- Protein metabolism by the salivary glands and other organs of the larva of the blowfly, Calliphora erythrocephalaJournal of Insect Physiology, 1974
- Maturation of the head of bacteriophage T4Journal of Molecular Biology, 1973