METHOD FOR THE ESTIMATION OF STRIATED-MUSCLE MASS IN SMALL LABORATORY-ANIMALS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 35  (2) , 215-218
Abstract
A method for the determination of the total muscle mass in small experimental animals is presented. Muscle proteins were quantified in the 1 M LiCl-soluble and distilled water-insoluble fraction of the eyeless, brainless, eviscerated and skinned carcass, as compared with a striated muscle sample from the same animal used as standard and processed in the same way as the whole carcass. The non-muscular tissues left in the carcass do not interfere with the final results. The total muscle mass in adult rats was estimated as 42.0 .+-. 2.8% of the in vivo weight.

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