Isolation of a gene encoding a novel spectinomycin phosphotransferase from Legionella pneumophila
- 1 June 1997
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 41 (6) , 1385-1388
- https://doi.org/10.1128/aac.41.6.1385
Abstract
A gene capable of conferring spectinomycin resistance was isolated from Legionella pneumophila, the agent of Legionnaires' disease. The gene (aph) encoded a 36-kDa protein which has similarity to aminoglycoside phosphotransferases. Biochemical analysis confirmed that aph encodes a phosphotransferase which modifies spectinomycin but not hygromycin, kanamycin, or streptomycin. The strain that was the source of aph demonstrated resistance to spectinomycin, and Southern hybridizations determined that aph also exists in other legionellae.Keywords
This publication has 25 references indexed in Scilit:
- Mechanism of aminoglycoside 3'-phosphotransferase type IIIa: His188 is not a phosphate-accepting residueChemistry & Biology, 1996
- Antimicrobial Chemotherapy for Legionnaires' Disease: A ReviewClinical Infectious Diseases, 1995
- Altered substrate specificity by substitutions at Tyr218 in bacterial aminoglycoside 3′-phosphotransferase-IIFEMS Microbiology Letters, 1992
- Interactions Between Macrophages and Legionella pneumophilaPublished by Springer Nature ,1992
- Mutations in the aphA-2 gene of transposon Tn5 mapping within the regions highly conserved in aminoglycoside-phosphotransferases strongly reduce aminoglycoside resistanceMolecular Microbiology, 1991
- Sensitivity and resistance of Legionella pneumophila to some antibiotics and combinations of antibioticsJournal of Antimicrobial Chemotherapy, 1988
- Nucleotide sequence of the ribostamycin phosphotransferase gene and of its control region in Streptomyces ribosidificusGene, 1988
- Nucleotide sequence analysis of a gene encoding a streptomycin/spectinomycin adenyltransferasePlasmid, 1985
- Nucleotide sequence of the kanamycin resistance transposon Tn903Journal of Molecular Biology, 1981
- Inactivation of beta-lactam antibiotics by Legionella pneumophilaAntimicrobial Agents and Chemotherapy, 1979