Comparison of [3H]Choline and d‐[3H]Aspartate Efflux from Guinea Pig and Human Neocortex

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Abstract
The outflow of [3H]choline ([3H]Ch) evoked by electrical field stimulation and the efflux of d‐[3H]Asp induced by 35 mM KCl and 1–10 μM ouabain were studied in human and guinea pig cortical slices, kept under identical experimental conditions. [3H]Ch outflow was significantly lower whereas d‐[3H]Asp efflux was significantly higher in humans than in guinea pigs. This suggests a different proportion of the two neuronal systems in these two species. Blockade of muscarinic autoreceptors with atropine increased, whereas stimulation of α2 receptors with norepinephrine (NE) reduced, the evoked [3H]Ch outflow to the same extent in human and guinea pig cortical slices. Conversely, NE did not affect ouabain‐induced d‐[3H]Asp efflux, suggesting that an α2‐mediated control is not operative in the glutamatergic cortical structures. Desmethylimipramine, 2–5 μM, was able to increase [3H]Ch outflow through atropine‐like mechanisms only in the human. This drug at 20–50 μM inhibited [3H]Ch and d‐[3H]Asp efflux in both species, through mechanisms unrelated to its monoamine reuptake blocking properties. Thus, similarities and differences can be detected between humans and guinea pigs with regard to (a) the relative potency of the cholinergic and acidic amino acidergic signals and (b) the modulation of neurotransmitter outflow by drugs acting on auto‐ and heteroreceptors.