Release of N2 ,3-ethanoguanine from haloethylnitrosourea-treated DNA by Escherichia coil 3-methyladenine DNA glycosylase II
- 1 October 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 12 (10) , 1971-1973
- https://doi.org/10.1093/carcin/12.10.1971
Abstract
3-Methyladenine DNA glycosylase II (Gly II), purified from Escherichia coli cells which carry the plasmid PYN1000, has been tested for its ability to release N2,3-ethanoguanine from DNA modified by the antitumor agent N-[2-chloroethyl-1,2-14C]-N'-cyclohexyl-Nnitrosourea ([14C]CCNU). Gly II has been shown to release N2,3-ethanoguanine in a protein-and thne-dependent manner at a rate that exceeds the rate at which this enzyme releases other alkylated bases from [14C]CCNU-modified DNA. This finding widens the known substrate specificity for Gly II to Include a modified base which bears an exocydic ring structure, a class of modifications caused by a variety of chemical carcinogens.Keywords
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