Abstract
An electrophoretic study of the self-fertilizing hermaphroditic fish, Rivulus marmoratus, revealed that it is completely homozygous for the 31 enzyme and protein determining gene loci examined. No differences were observed between strains from Florida (U.S.A.) and Curacao (Netherlands Antilles); however, strains from Cuzomel Island (Mexico) differed at four loci. The evolutionary consequences of selfing are discussed with regard to the natural history of this fish.