CYTOPHOTOMETRIC DETERMINATION OF THE EFFECTS OF X-IRRADIATION ON PROTEIN-BOUND SULFHYDRYL AND DISULFIDE GROUPS
Open Access
- 1 July 1959
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 7 (4) , 267-270
- https://doi.org/10.1177/7.4.267
Abstract
In the exteriorized rat kidney irradiated with 2000 r, no radiation induced changes in concentration of cytoplasmic protein-bound sulfhydryl (SH) or disulfide (SS) groups were seen in the tubule cells during the 5 days following irradiation. In the small intestine, histological responses were observed 20 hours following 750 r whole body irradiation, but there were no significant changes (at the 0.01 level) in SH or SS concentrations. At 5 days post-irradiation, there was a significant change only in the striate-bordered villus epithelial cells of the duodenum, where SH concentration decreased. This was the only area that showed prominent degenerative changes. It appears that X-irradiation has no histochemically demonstrable primary effect on cytoplasmic protein-bound SH or SS concentrations; in fact, definite morphological responses to irradiation occur without changes in concentration of these groups. A decrease in SH concentration occurs only when secondary degenerative changes supervene.Keywords
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