IA-ANTIGEN EXPRESSION AND IL-1 ACTIVITY IN MURINE TUMOR-ASSOCIATED MACROPHAGES

  • 1 December 1986
    • journal article
    • research article
    • Vol. 59  (4) , 527-533
Abstract
Tumour-associated macrophages (TAM) isolated from five murine sarcomas had a relatively high frequency of I-A+ cells, with mean values of 27% (mFS6), 52% (MN/MCA1), 68% (N3), 62% (N4) and 98% (J3) for TAM compared to 12% for resident peritoneal macrophages. Expression of I-E in TAM was also high (29%) in the only sarcoma (N4) examined in this respect. Expression of I-A by TAM declined in culture but exposure to lymphokine supernatants maintained and increased the frequency of I-A+ cells in TAM. Transplantation of tumours into nude mice caused a marked decrease in the percentage of I-A+ TAM in the case of the N4 sarcoma (8% compared to 48%), whereas for the MN/MCA1 sarcoma the diminution was only marginal (from 53 to 41%). TAM from murine sarcomas did not constitutively release appreciable levels of interleukin-1 (IL-1) activity. Upon stimulation with bacterial lipopolysaccharides or silica. TAM showed a limited capacity to produce and release LH-1 activity compared to peritoneal macrophages. Thus the expression of I-A antigens and the IL-1-producing capacity are uncoupled in TAM from murine sarcomas. These properties of TAM could play an important role in the generation of anti-tumor immunity and/or of suppressive T-cell circuits.