Joint molecules of λ DNA as an intermediate of genetic recombination

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Abstract
Joint molecules of λ DNA formed in the absence of DNA replication, which may be involved in the process of genetic recombination can be observed as branched DNA derived from different phage particles. These molecules are associated through base-pair hydrogen bonding in synaptic regions, usually with short single-stranded gaps. Furthermore, joint molecules could be accumulated up to ten fold when λ was irradiated with ultraviolet light before infection ofpolI mutant ofE. coli. Infection at low multiplicity did not give rise to joint molecules. These results suggest that single-strand breaks and gaps introduced in duplex λ DNA facilitate the formation of joint molecules.