The Effect of Freezing on Encysted Anisakis Larvae

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Abstract
Immature encysted Anisakis sp. from commercial fish from Puget Sound were exposed to various freezing temps. to determine resistance to cold. At -5[degree] and -10[degree]C. most of the nematodes died in the first 24 hrs.; the few remaining survivors may remain alive for several days. At -17[degree]C, all died within 24 hrs. At -30[degree]C, death apparently occurs within 5 min. Commercial freezing of herring in 100-lb. blocks using initial temp. of -30[degree]C for 16 hrs. and storage at -12[degree]C will kill all Contracaecum larvae and nearly all of the Anisakis larvae in the 1st 24 hrs. The few survivors die within the 1st week.

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