Studies of esterase 6 inDrosophila melanogaster: XIV. Variation of esterase 6 levels controlled by unlinked genes in natural populations

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Abstract
SUMMARY: The esterase 6 (Est-6) locus inDrosophila melanogasteris located on the third chromosome and is the structural gene for a carboxylesterase (E.C.3.1.1.1) and is polymorphic for two major electromorphs (slow and fast). Isogenic lines containingXchromosomes extracted from natural populations and substituted into a common genetic background were used to detect unlinked factors that affect the activity of theEst-6locus. Twofold activity differences of esterase 6 (EST 6) were found among males from these derived lines, which differ only in theirXchromosome. These unlinked activity modifiers identify possible regulatory elements. Immunoelectrophoresis was used to estimate quantitatively the levels of specific cross-reacting material in the derived lines. The results show that the variation in activity is due to differences in the amount of EST 6 present. The data are consistent with the hypothesis that there is at least one locus on the X chromosome that regulates the synthesis of EST 6 and that this regulatory locus may be polymorphic in natural populations.