Unscheduled synthesis of DNA during mammalian spermatogenesis in response to UV irradiation

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Abstract
Unscheduled incorporation of tritium‐labeled thymidine into nuclear DNA, induced by UV irradiation and presumed to indicate DNA repair replication, has been followed in differentiating male germ cells of the rat and rabbit by quantitative autoradiographic techniques. Cells at various stages of differentiation incorporated [3H] thymidine at different rates. The maximal rate was observed in nuclei of pachytene primary spermatocytes; the rate was markedly lower in spermatogonia and in leptotene and zygotene primary spermatocyte nuclei. No evidence of incorporation was found for nuclei of spermatids and testis spermatozoa.