Insulin Receptor Protein Rendered Visible in Triton X-114 Membranes
- 1 January 1985
- journal article
- research article
- Published by Walter de Gruyter GmbH in Biological Chemistry Hoppe-Seyler
- Vol. 366 (2) , 695-698
- https://doi.org/10.1515/bchm3.1985.366.2.695
Abstract
Insulin receptor protein [porcine] was visualized by EM. This was rendered possible after the molecules were extensively purified and were reinserted into Triton X-114 membranes in a situation similar to that found in biological membranes. Freeze fracturing then revealed the insulin receptor molecules as particles randomly distributed in the fracture plane of the artificial membranes. The sizes of the particles are in the range expected from biochemical data.This publication has 14 references indexed in Scilit:
- A critical interpretation of experiments of binding of peptide hormone to specific receptors by computer modellingBiochimica et Biophysica Acta (BBA) - General Subjects, 1982
- Purification of the Insulin Receptor Protein from Porcine Liver MembranesHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1981
- Structural details of membrane-bound acetylcholine receptor from Tropedo marmorata.Proceedings of the National Academy of Sciences, 1980
- Insulin receptor: covalent labeling and identification of subunits.Proceedings of the National Academy of Sciences, 1979
- Hydrophobic membrane protein from chromatophores of Rhodospirillum rubrum. Structural and spectroscopic studies of monolayers and multilayersBiochimica et Biophysica Acta (BBA) - Biomembranes, 1979
- Structural changes during the transformation of bacteriophage T4 polyheads: Characterization of the initial and final states by freeze-drying and shadowing fab-fragment-labelled preparationsJournal of Molecular Biology, 1978
- Molecular structure determination by electron microscopy of unstained crystalline specimensJournal of Molecular Biology, 1975
- Affinity Chromatography and Purification of the Insulin Receptor of Liver Cell MembranesProceedings of the National Academy of Sciences, 1972
- Isolation of the Insulin Receptor of Liver and Fat-Cell MembranesProceedings of the National Academy of Sciences, 1972