Optimization of 1,3‐propanediol production by novel recombinant Escherichia coli using response surface methodology
- 24 April 2006
- journal article
- research article
- Published by Wiley in Journal of Chemical Technology & Biotechnology
- Vol. 81 (6) , 1075-1078
- https://doi.org/10.1002/jctb.1538
Abstract
Response surface methodology was used to optimize 1,3‐propanediol production by a novel recombinant Escherichia coli JM109 (pHsh‐dhaB‐yqhD). The optimal fermentation parameters for enhanced 1,3‐propanediol yield were found to be: glycerol 61.8 g L−1, yeast extract 6.2 g L−1, Vitamin B12 0.049 g L−1 and fermentation time 30 h. Subsequent experimental trials confirmed the validity of the model. These optimal fermentation conditions in the cultivation flask culture led to a 1,3‐propanediol concentration of 43.1 g L−1 and a conversion rate of 69.7% (g g−1). A maximum 1,3‐propanediol concentration of 41.1 g L−1 was achieved in a 5 L fermenter using the optimized parameters. Copyright © 2006 Society of Chemical IndustryKeywords
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