Role of Vitamin B 12 in Methyl Transfer for Methane Biosynthesis by Methanosarcina barkeri
- 16 April 1982
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 216 (4543) , 303-305
- https://doi.org/10.1126/science.7063887
Abstract
When Methanosarcina barkeri is grown on methanol as the sole carbon source, a B12-containing protein is synthesized by this organism. This B12 protein contains bound aquocobalamin, and when this cofactor is reduced and methylated with [14C]methyl iodide, the resultant [14C]methyl B12 protein is extremely active in the biosynthesis of 14C-labeled methane. These findings indicate that a B12-dependent system is operative in the biological formation of methane in addition to other systems that are B12-independent.This publication has 5 references indexed in Scilit:
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- Formation of Methane from Serine by Cell-free Extracts of Methanobacillus omelianskiiJournal of Biological Chemistry, 1965