Properties of a Serologically Active Substance from Leptospira icterohemorrhagiae (Wijnberg)

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Abstract
Suspensions of viable cells of L. icterohemorrhagiae were disrupted with an equal vol. of a soln. containing 0.1 [image] NaCl, 0.2 [image] Na citrate, and 0.4% Na desoxycholate. An immunologically-reactive, non-dialyzable substance (Fraction 1) was prepd. by a procedure which avoided heating and use of alkalis and acids. The active principle was isolated in mixture with a non-dialyzable polynucleotide moiety. The organism of L. icterohemorrhagiae contained an appreciable amt. of the polynucleotide substance and was composed of pentose- as well as desoxypentosenucleic acids. Crystalline ribo- and desoxyribonuclease altered the absorption spectrum of the nucelotide, but failed to inactivate the antigen. On the basis of the latter evidence it appeared likely that the polynucleotide was not the determinant of immunological reactivity. The active principle resisted the action of trypsin, hemicellulase was not destroyed by heating for 1 hr. at 100[degree]C.

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