DIFFERENTIATION OF URINARY CORTICOSTEROIDS ACCORDING TO THEIR OXYGEN CONTENT

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Abstract
SUMMARY: An analytical method was developed which comprises the following successive operations: enzymic hydrolysis of urinary steroid conjugates; extraction and initial purification; reduction with potassium borohydride; paper-chromatographic separation into three fractions containing corticosteroid triols, tetrols and pentols, respectively; measurement of 17-hydroxycorticosteroids and of 17-deoxycorticosteroids in each fraction. Subject to certain qualifications the outlined procedure is believed to account separately for the metabolites of cortisol, 17α-hydroxyprogesterone, corticosterone and 11-deoxycorticosterone; it fails to distinguish between the metabolites of 21-deoxycortisol and 11-deoxycortisol. Assays of urines from non-pregnant women, pregnant women and newborn children revealed a different pattern in the composition of urinary corticosteroids in each group of subjects.

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