In Vitro Production of Granular T Cells and Mast Cells by Use of Different Conditioned Media.

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Abstract
Normal mouse spleen cells were cultured in different conditioned media (CM). Mixed lymphocyte culture supernatant (MLC SN) was shown to promote the proliferation of cytotoxic, Thy-l+, Lyt-1+, Lyt-2+, asialo-GM-1 +, weakly adherent cells with numerous vacuoles and lysosomc-like cytoplasmic granules. In contrast, the Con A SN induced the proliferation of non-cylotoxic, Thy-1−. Lyt−1−, Lyt-2−, asialo-GM-I, non-adherent cells with numerous cytoplasmic granules. The ultrastructural morphology of these cells and the cytochemical characteristics of their granules enable us to identify them as mast celts. The different effects of both CM could be related to their T-cell growth factor (TCGF) content. When the amount of TCGF of these two CM was determined (by assaying growth-stimulating activity for T-cell colonies), it appeared that the MLC SN contained larger amounts of TCGF (han the Con A SN used in these experiments.

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