Different Mechanisms Protect Transgenic Tobacco Against Tomato Spotted Wilt and Impatiens Necrotic Spot Tospoviruses

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Abstract
We generated transgenic tobacco plants expressing the sense or antisense untranslatable N coding sequence of the lettuce isolate of tomato spotted wilt virus (TSWV-BL) as well as transgenic plants containing the promoterless N gene of the virus. Both sense and antisense untranslatable N gene RNAs provided protection against homologous and closely related isolates but not against distantly related Tospoviruses. These RNA-mediated protections were most effective in plants that synthesized low levels of the respective RNA species and appears to be achieved through the inhibition of viral replication. Unlike the sense RNA-mediated protection, the level of the antisense RNA-mediated protection depended on the concentration of the inoculum and the size of the test plants. Comparisons with previous results in transgenic plants expressing the intact N gene suggest that resistance to homologous and closely related TSWV isolates in plants that express low levels of the translatable N gene is due to the presence of the N gene transcript and not the N protein. In contrast, resistance to distantly related Tospoviruses is due to accumulation of high levels of the N protein and not due to the presence of the N gene transcript.