Repair of O6-methylguanine, by mammalian cell extracts, in alkylated DNA and poly(dG-m5 dC).(poly dG-m5 dC) in B and Z forms
- 1 January 1985
- journal article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 6 (5) , 805-807
- https://doi.org/10.1093/carcin/6.5.805
Abstract
The ability of rat hepatoma cells extracts to repair O6-methylguanine was measured using as substrate alkylated DNA and alkylated poly(dG-m5 dC).poly(dG-m5 dC) either in the B or in the Z conformation. The O6-methylguanine-DNA methyltransferase had similar activities on DNA and on the right-handed polymer. However, when the polymer was in the Z conformation, the protein activity represented ∼10% of that measured on DNA. Therefore the efficiency of O6-methylguanine repair is related to the DNA conformation and is markedly reduced during the B to Z transition of the substrate.Keywords
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