The construction and cloning of synthetic genes coding for artificial proteins and expression studies to obtain fusion proteins
- 1 January 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Protein Engineering, Design and Selection
- Vol. 1 (4) , 345-351
- https://doi.org/10.1093/protein/1.4.345
Abstract
Synthetic genes coding for artificial proteins with predeflned and nutritionally valuable amino acid compositions have been constructed and cloned In bacterial plasmid vector pKK233-2. The genes were constructed from three easily interchangeable ‘cassettes’ encoding either essential, non-essential or branched-chain amino acid residues. A potential hairpin loop structure in the mRNA around the region of the ribosome binding site was probably the reason for blockage of translation from this vector. Two selected genes, AHB (containing one copy of each cassette) and A (consisting of six copies concatemerized A6cassette) were cloned into pUR300, a (β-Gal fusion vector and expressed as fusion proteins (β-Gal-AHB and (β-Gal-A6.This publication has 15 references indexed in Scilit:
- Studies on transformation of Escherichia coli with plasmidsPublished by Elsevier ,2006
- An alternate method for synthesis of double-stranded DNA segments.Journal of Biological Chemistry, 1982
- Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genesGene, 1982
- Systematic alteration of the nucleotide sequence preceding the translation initiation codon and the effects on bacterial expression of the cloned SV40 small-t antigen geneGene, 1982
- Total synthesis of a human leukocyte interferon geneNature, 1981
- Secondary structure of mRNA and efficiency of translation initiationGene, 1980
- Design, synthesis and characterisation of a 34-residue polypeptide that interacts with nucleic acidsNature, 1979
- Total Synthesis of a GeneScience, 1979
- Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.Proceedings of the National Academy of Sciences, 1977
- Triplet information in helix prediction applied to the analysis of super-secondary structuresJournal of Molecular Biology, 1977