Purification and characterization of phosphatidylinositol 4-phosphate 5-kinases
- 1 December 1992
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 288 (2) , 637-642
- https://doi.org/10.1042/bj2880637
Abstract
We detail the purification and characterization of three distinct isoforms of PtdIns4P 5-kinase present in bovine brain. One of these, PtdIns4P 5-kinase C, was purified to apparent homogeneity, and SDS/PAGE analysis demonstrated a single polypeptide and molecular mass 53 KDa. These three isoforms were shown to differ in their kinetic properties, and immunological characterization with an antibody raised to PtdIns4P 5-kinase C demonstrated that this isoform was unrelated to the other two. Furthermore, PtdIns4P 5-kinase C was shown to be the bovine brain homologue of the Type II PtdIns4P 5-kinase previously purified from human erythrocytes [Bazenet, Ruano, Brockman & Anderson (1990) J. Biol. Chem. 265, 18012-18022].Keywords
This publication has 25 references indexed in Scilit:
- Pathway of phosphatidylinositol(3,4,5)-trisphosphate synthesis in activated neutrophilsNature, 1991
- Purification and partial characterization of phosphatidylinositol-4-phosphate kinase from rat liver plasma membranes. Further evidence for a stimulatory G-proteinBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1990
- Polyphosphoinositide synthesis in platelets stimulated with low concentrations of thrombin is enhanced before the activation of phospholipase CFEBS Letters, 1990
- Purification of a Phosphatidylinositol 4‐Phosphate Kinase from Bovine Brain MembranesJournal of Neurochemistry, 1990
- Modulation of the activity of purified phosphatidylinositol 4-phosphate kinase by phosphorylated and dephosphorylated B-50 proteinBiochemical and Biophysical Research Communications, 1985
- Breakdown and synthesis of polyphosphoinositides in fMetLeuPhe‐stimulated neutrophilsFEBS Letters, 1985
- Mitogens increase phosphorylation of phosphoinositides in thymocytesNature, 1984
- Fertilization increases the polyphosphoinositide content of sea urchin eggsNature, 1984
- Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphateNature, 1983
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979