Relationships between a new type IV secretion system and the icm/dot virulence system of Legionella pneumophila
- 1 November 1999
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 34 (4) , 799-809
- https://doi.org/10.1046/j.1365-2958.1999.01642.x
Abstract
We describe here a Legionella pneumophila type IV secretion system that is distinct from the previously described icm/dot system. This type IV secretion system contains 11 genes (lvh ) homologous to genes of other type IV secretion systems, arranged in a similar manner. The lvh genes were found to be located on a DNA island with a GC content higher than the L. pneumophila chromosome. In contrast to the icm/dot system that was shown to be required for intracellular growth in HL‐60‐derived human macrophages and Acanthamoeba castellanii, the lvh system was found to be dispensable for intracellular growth in these two hosts. The lvh system was found to be partially required for RSF1010 conjugation, a process that was previously shown to be completely dependent on several icm/dot genes. However, results obtained from analysis of double mutants in the icm/dot genes and the lvh genes revealed that lvh genes can substitute for some components of the icm/dot system for RSF1010 conjugation, but not for intracellular growth. These results indicate that components of the icm/dot system and components of the lvh type IV secretion system are able to interact with one another.Keywords
This publication has 73 references indexed in Scilit:
- The Legionella pneumophila PilT Homologue DotB Exhibits ATPase Activity That Is Critical for Intracellular GrowthJournal of Bacteriology, 2004
- How is the intracellular fate of the Legionella pneumophila phagosome determined?Trends in Microbiology, 1998
- Altered intracellular targeting properties associated with mutations in the Legionella pneumophila dotA geneMolecular Microbiology, 1994
- Common ancestry between IncN conjugal transfer genes and macromolecular export systems of plant and animal pathogensMolecular Microbiology, 1994
- Mutagenesis of Legionella pneumophila using Jn903 dllIacZ: identification of a growth‐phase‐regulated pigmentation geneMolecular Microbiology, 1994
- Common components in the assembly of type 4 fimbriae, DNA transfer systems, filamentous phage and protein‐secretion apparatus: a general system for the formation of surface‐associated protein complexesMolecular Microbiology, 1993
- Characterisation of a Pseudomonas aeruginosa twitching motility gene and evidence for a specialised protein export system widespread in eubacteriaGene, 1991
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Legionnaires' Disease Bacterium (Legionella pneumophila) Multiplies Intracellularly in Human MonocytesJournal of Clinical Investigation, 1980
- RESPONSE OF CULTURED MACROPHAGES TO MYCOBACTERIUM TUBERCULOSIS, WITH OBSERVATIONS ON FUSION OF LYSOSOMES WITH PHAGOSOMESThe Journal of Experimental Medicine, 1971