Double-Staining Methods at the Ultrastructural Level Applying Colloidal Gold Conjugates
- 1 January 1988
- journal article
- research article
- Published by Taylor & Francis in Ultrastructural Pathology
- Vol. 12 (3) , 279-290
- https://doi.org/10.3109/01913128809098040
Abstract
Several double gold-labeling methods have been reviewed, and our own results of applying some of these methods have been included. Of the six double-staining methods tested, four gave satisfactory results (indirect double immunogold staining method, two-face protein A-gold staining method, two-face amplified protein A-gold staining method, and formaldehyde blockade protein A-gold staining method). The other two techniques (sequential double protein A-gold staining method and formaldehyde blockade amplified protein A-gold staining method) showed cross-reaction between the first and second staining sequence.Keywords
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