Some Characteristics of the Estradiol Binding Protein of the Mature, Intact Rat Uterus
- 1 November 1971
- journal article
- other
- Published by The Endocrine Society in Endocrinology
- Vol. 89 (5) , 1270-1279
- https://doi.org/10.1210/endo-89-5-1270
Abstract
In vivo accumulation of 3H-estradiol by the uterus of the intact rat, its distribution between the 105,000 × g supernatant and particulate fractions, and the in vitro binding of 3H-estradiol to the 8S receptor of the uterine cytosol, were determined at denned stages of the estrous cycle. In vitro binding of 3H-estradiol to the 8S receptor was minimal on the day of metestrus and maximal on the day of estrus. The total in vivo accumulation of estradiol in picomoles per uterus was 1.32 on the day of metestrus (L1); 2.00 on diestrus (L2); 2.16 on proestrus (O1); and 1.63 on estrus (C0). Subcellular estradiol distribution was essentially identical on days C0, L1, and L2; on day O1 there was a change in distribution in favor of the particulate fraction. It was inferred that net synthesis of estrogen receptor occurs between day L1 and L2 of the cycle and probably continues to the morning of proestrus. It was also observed that the uterus of the metestrous and diestrous animal was refractive to administered estrogen. (Endocrinology89: 1270, 1971)This publication has 0 references indexed in Scilit: