IMMUNOLOGICAL AND BIOCHEMICAL-EVIDENCE FOR IDENTITY OF TARTRATE-RESISTANT ISOENZYMES OF ACID-PHOSPHATASES FROM HUMAN-SERUM AND TISSUES

  • 1 January 1980
    • journal article
    • research article
    • Vol. 26  (3) , 420-422
Abstract
Acid phosphatase isoenzyme 5b was purified from a human spleen affected by leukemic reticuloendotheliosis and used to produce a specific antiserum. The antiserum was used to show complete immunological identity among isoenzymes 5a and 5b in human serum, and 5b isolated from a giant-cell bone tumor and from the spleen of a case of Hodgkin''s disease. Acid phosphatase 5b in a giant-cell bone tumor was isolated for biochemical characterization. Its pH optimum and substrate specificity were very similar to those of isoenzyme 5b from human spleen.