CHARACTERIZATION AND SEQUENCING OF THE REGION CONTAINING GENE-N, THE NUTL SITE AND TL1 TERMINATOR OF BACTERIOPHAGE -PHI-80
- 1 January 1985
- journal article
- research article
- Vol. 38 (1-3) , 119-129
Abstract
We have cloned the early region of the major leftward operon of bacteriophage .vphi.80 DNA and determined the nucleotide sequence of the pL-nutL-tL1 segment. The genes and sites equivalent to .lambda. gene N, antiterminator nutL, and terminator tL1 were found in this region by functional tests using the recombinant plasmids carrying the transcriptional units Ptrp-tL1-galK, Ptrp-nutL-tL1-galK and PlacUV5-N. Sequence homologies were observed between the boxA of the phage 21 nutR site and the inverted repeat of the nut core in the nutL-equivalent site of .vphi.80. The N-equivalent gene of .vphi.80 codes for a protein with 98 amino acids. It is highly basic as is the .lambda. N protein. Recombinant plasmids carrying the .vphi.80-pL promoter and nut-equivalent site of .vphi.80 are lethal to the Escherichia coli host cell, but can be stably maintained in the presence of the .vphi.80-cI repressor.This publication has 14 references indexed in Scilit:
- Origin of replication of Escherichia coli plasmid RSF 1030Molecular Genetics and Genomics, 1982
- Antitermination and termination functions of the cloned nutL, N and tL1 modules of coliphage lambdaGene, 1981
- Interaction of the sigma factor and the nusA gene protein of E. coli with RNA polymerase in the initiation-termination cycle of transcriptionCell, 1981
- Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H.Proceedings of the National Academy of Sciences, 1980
- The N protein of bacteriophage lambda, defined by its DNA sequence, is highly basicGene, 1979
- Coliphage λnutL−: A unique class of mutants defective in the site of gene N product utilization for antitermination of leftward transcriptionJournal of Molecular Biology, 1978
- Transcription termination sites in the major leftward operon of coliphage lambdaVirology, 1978
- The relationship between function and DNA sequence in an intercistronic regulatory region in phage λNature, 1978
- Restriction endonuclease mapping of the Escherichia coli K12 chromosome in the vicinity of the ilv genesJournal of Molecular Biology, 1977
- Suppressor-sensitive mutants of coliphage ∅80Virology, 1968