Immunohistochemical Colocalization of GABA and Insulin in β-Cells of Rat Islet

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Abstract
γ-Aminobutyric acid (GABA) is found in high concentrations in the pancreatic islet. In addition, enzymes regulating the level of GABA (L-glutamate decarboxylase and GABA-α-ketoglutarate transaminase) have been immunohistochemically localized in the medullary cells of the islet. In this study, an immunofluorescence and elution/restaining protocol is used to determine the distribution of GABA and either insulin, glucagon, or somatostatin in a tissue section. GABA was not detected within the islet α- or δ-cells but was determined to be localized within the insulin-containing β-cells.

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