Ultrastructural Effects of Recombinant Gamma-Interferon on Cultured Human Keratinocytes

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Abstract
To extend our initial observations that recombinant gamma-interferon (r γIFN) influences the growth and differentiation of normal cultured human keratinocytes, we studied the electron microscopic changes induced by r γIFN. Treatment of cultured human keratinocytes with 103 units/ml (7.1 nM) of r γIFN produced a shift toward an increasing percentage of attached cells that had a mature-type, differentiated appearance rather than a basal type of proliferating cell, as observed in control, untreated cultures. This report extends the number of cell types that can be influenced by r γIFN at the ultrastructural level and supports the notion that r γIFN can influence both growth and maturation of normal cultured human keratinocytes.