Preparation, Properties and Gas Chromatography of the N-Trifluoroacetyl Esters of the Amino Acids

Abstract
The synthesis and properties of the trifluoroacetyl methyl esters of all of the common amino acids are described. Both the mono- and di-acetylated derivatives of cysteine, histidine, hydroxyproline, serine, threonine, tryptophan, and tyrosine were prepared and their comparative properties in gas chromatography studied. The conditions for the resolution of all of the common amino acid derivatives by gas-liquid chromatography and an apparatus to accomplish this in a period of from ½ to one hour is described.

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