The MAP kinase signal transduction pathway is activated by the endogenous cannabinoid anandamide
- 13 February 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 359 (2-3) , 133-136
- https://doi.org/10.1016/0014-5793(95)00027-7
Abstract
Anandamide is an endogenous ligand for delta 9-tetrahydrocannabinol (THC) receptors. Incubation of cultured cells with anandamide or THC causes increased arachidonic acid release and eicosanoid biosynthesis. Here we demonstrate that the MAP kinase signal transduction pathway contributes to this response. Treatment of WI-38 fibroblasts with anandamide causes increased MAP kinase activity and increased phosphorylation of the arachidonate-specific cytoplasmic phospholipase A2 (cPLA2). Significantly, MAP kinase phosphorylates and activates cPLA2 [Lin, et al., Cell, 72 (1993) 269-278]. The MAP kinase signal transduction pathway may therefore mediate the effects of anadamide on cPLA2 activation and arachidonic acid release.Keywords
This publication has 22 references indexed in Scilit:
- Control of MAP kinase activation by the mitogen-induced threonine/tyrosine phosphatase PAC1Nature, 1994
- Molecular characterization of a peripheral receptor for cannabinoidsNature, 1993
- Anandamide, a Brain Endogenous Compound, Interacts Specifically with Cannabinoid Receptors and Inhibits Adenylate CyclaseJournal of Neurochemistry, 1993
- cPLA2 is phosphorylated and activated by MAP kinaseCell, 1993
- Isolation and Structure of a Brain Constituent That Binds to the Cannabinoid ReceptorScience, 1992
- G-protein mediation of cannabinoid-induced phospholipase activationPharmacology Biochemistry and Behavior, 1991
- A novel arachidonic acid-selective cytosolic PLA2 contains a Ca2+-dependent translocation domain with homology to PKC and GAPCell, 1991
- A G Protein Mutant That Inhibits Thrombin and Purinergic Receptor Activation of Phospholipase A 2Science, 1990
- Structure of a cannabinoid receptor and functional expression of the cloned cDNANature, 1990
- The central neuropharmcology of psychotropic cannabinoidsPharmacology & Therapeutics, 1988