An Improved Immunoperoxidase Technique Using Horseradish Peroxidase Avidin D

1 February 1989

journal article
Published by Oxford University Press (OUP) in Laboratory Medicine

Vol. 20 (2) , 109-112
https://doi.org/10.1093/labmed/20.2.109

Abstract

We have compared the avidin-biotin complex immunoperoxidase method with a modified horseradish peroxidase a vidin D technique. Use of the horseradish peroxidase avidin D procedure saved time without compromising quality of the staining, by reducing the amount of time for methanol-hydrogen peroxide block, serum block, antibody incubations, horseradish peroxidase avidin D incubation, and Gill-3 hematoxylin counterstain, and cut costs by using higher dilutions of primary antibodies. Other helpful modifications include the utilization of phosphate-buffered saline, batching slides for secondary antibody and horseradish peroxidase avidin D, and instituting a standard development time.

Keywords

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