RADIOIMMUNOSCINTIGRAPHY OF HUMAN-COLON CANCER XENOGRAFTS IN MICE WITH RADIOIODINATED MONOCLONAL-ANTIBODY B72.3

  • 1 January 1984
    • journal article
    • research article
    • Vol. 25  (11) , 1197-1203
Abstract
Monoclonal antibody B72.3 reacts with a tumor-associated that is found on human breast and colon carcinomas in significantly higher concentration than in normal adult tissues. Intact B72.3 IgG was labeled with 131I or 125I and injected into athymic mice bearing xenografts of human colon cancer. Whole-body scintiphotos obtained with a pinhole collimator demonstrated tumor localization within 24 h after i.v. administration, and the tumor-to-background ratio rose continuously for at least 14 days. Progressive antibody accumulation was observed in the tumor during the first 3 days, but no significant normal organ localization was observed at any time. No localization was seen in control tumors, a human melanoma xenograft that lacks the antigen recognized by B72.3. The pharmacokinetics of this antibody in tumor-bearing mice suggest that 131I B72.3 may be useful for radioimmunotherapy as well as radioimmunoscintigraphy of colon cancer in man.